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1994-10-25
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Document 2762
DOCN M94A2762
TI Evaluation of saliva test in HIV infection in Rosario, Argentina.
DT 9412
AU Taborda M; Fuster L; de Rueda M; Bortolozzi R; Lupo S; Fay O; Center for
technology in Public Health, National University of; Rosario, Argentina.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):230 (abstract no. PB0351). Unique
Identifier : AIDSLINE ICA10/94369813
AB OBJECTIVE: To evaluate diagnostic usefulness of a saliva collection
device detecting HIV 1-2 antibody. To compare the sensibility and
specificity obtained between samples of saliva and serum. To know the
relation between HIV 1-2 Ab presence or absence in saliva and clinical
status HIV infected patients. MATERIAL AND METHODS: We were able to
study 148 adult patients. Group I were 90 (61%) HIV(+) patients with
different clinical status and group II were 58 (39%) people with
spontaneous presentation to our Center in order to know their
serological status. In 20 (22%) patients with AIDS from group I we were
able to collect once a month during 3 months saliva and blood samples.
Omni-sal (Saliva Inc. M.R.) were used to obtain saliva samples. Organon
Teknika and Abbott laboratory Elisa test were used for screening and
Western blot and LiaTek from Organon were used for confirmatory test. We
follow the changes suggested by the manufacturer for the saliva samples
process (sample volume, a-IgA peroxidase conjugate). RESULTS: In the
group I the saliva and serum correlation was 96,4% (87(+)/90(+)). 10
(11.49%) out of 87 EIA(+) samples were Western blot indeterminate(we
used the ARC criteria of positivity) showed generally anti-env Antibody.
3(-) saliva samples were indeterminate Western blot (showed gp41 gp120
gp160) and correspond with the AIDS patient 3th samples. In the group II
patients, the saliva and serum correlation were 96.5% (8(+)/10(+);
48(-)/48(-)). The two negative samples were indeterminate in serum
(Western blot (1) gp41 gp120 gp160 (2) p24) and HIV(+) partners with
seroconversion. CONCLUSIONS: The test shows a low sensitivity in the
adult population tested. 2) The mayor differences have been detected
during the seroconversion period. 3) In AIDS patients with low
antibodies titles, whereas Western blot remain indeterminate, saliva
test became negative. 4) More extensive study must be done, particularly
regarding screening among at risk population.
DE Adult Argentina/EPIDEMIOLOGY Blotting, Western Comparative Study
Enzyme-Linked Immunosorbent Assay Evaluation Studies Human HIV
Antibodies/*ANALYSIS/BLOOD HIV Infections/*DIAGNOSIS/EPIDEMIOLOGY
HIV-1/*IMMUNOLOGY HIV-2/*IMMUNOLOGY Sensitivity and Specificity
MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).